Email updates

Keep up to date with the latest news and content from Diagnostic Pathology and BioMed Central.

This article is part of the supplement: New trends in digital pathology: Proceedings of the 9th European Congress on Telepathology and 3rd International Congress on Virtual Microscopy

Open Access Open Badges Proceedings

Object orientated automated image analysis: quantitative and qualitative estimation of inflammation in mouse lung

Coralie Apfeldorfer*, Kristina Ulrich, Gareth Jones, David Goodwin, Susie Collins, Emanuel Schenck and Virgile Richard

Diagnostic Pathology 2008, 3(Suppl 1):S16  doi:10.1186/1746-1596-3-S1-S16

PubMed Commons is an experimental system of commenting on PubMed abstracts, introduced in October 2013. Comments are displayed on the abstract page, but during the initial closed pilot, only registered users can read or post comments. Any researcher who is listed as an author of an article indexed by PubMed is entitled to participate in the pilot. If you would like to participate and need an invitation, please email, giving the PubMed ID of an article on which you are an author. For more information, see the PubMed Commons FAQ.

On number of sections and statistical power

Vincenzo Della Mea   (2009-02-10 09:18)  University of Udine, Italy

After having attended the Authors' congress presentation, I read the paper with much interest, because it depicts very well a protocol for obtaining reliable results from image analysis on histological sections.

However I would like to point out that the section entitled "Number of sections has little influence on statistical power of the study" reports a result well known since many years: statistical accuracy of a study depends more on the number of subjects enrolled than on the number of samples within the same subject, according to the Gundersen expression “Do more less well” (1). When dealing with rare condition, taking more samples from few subjects seems a shortcut for obtaining statistically significant results, but is not always the case.

(1) Gundersen HJG, Osterby R. Optimizing sampling efficiency of stereological studies in biology: or “do more less well!”. J Microsc 1981; 121-65.

Competing interests



Post a comment