Table 1

Stain

Number of images

Distance shading

Distance grey value distribution

Distance grey value height

H & E

1400

49

54

46

DAB

1200

32

39

35

AP

400

38

42

39

DAPI & TEXAS RED & FITC

120

98

75

129

H & E: hematoxilin – eosin; DAB: diaminobencidine; AP: Alcalic phosphatase; DAPI & Texas Red & FITC: tri-colour fluorescent stain

Kayser et al. Diagnostic Pathology 2008 3(Suppl 1):S11   doi:10.1186/1746-1596-3-S1-S11