Detection of Her-2/neu gene expression in human breast cancer tissue by FISH analysis. Two exemplary tissues of breast cancer exhibiting no amplification (A, B) or strong amplification of Her-2/neu gene (C, D) are shown (400× magnification). ZyGreen labeled Her-2/neu gene signals captured by advanced Nikon fluorescent microscope in combination with conventional 100W mercury vapor lamp (A, C) are directly compared with those captured by standard bright field Carl Zeiss Axiostar Plus microscope equipped with a Fraen AFTER LED Fluorescence Microscope Kit (B, D). Cerb-B2 gene expression was detected by a 480 nm Fluorescence light cassette combined with a 510 nm long pass emission filter mounted to the Axiostar microscope. Equal quality of the captured pictures was achieved by integration times between 1 and 3 seconds (A, C, 100W mercury vapor) and 6 and 10 seconds (B, D, LED).
Lang et al. Diagnostic Pathology 2008 3:49 doi:10.1186/1746-1596-3-49