Brightfield double in situ hybridization (BDISH) signal detection scheme with a sequential in situ hybridization method. HER2 gene signal was detected with a DNP-labeled nick translated DNA probe hybridization followed by silver signal detection system (silver acetate, hydroquinone, and H2O2 reaction). Then, chromosome 17 centromere (CEN 17) signal was detected with a DNP-labeled CEN 17 oligoprobe hybridization followed by fast red and naphthol phosphate reaction signal detection system.
Nitta et al. Diagnostic Pathology 2008 3:41 doi:10.1186/1746-1596-3-41