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This article is part of the supplement: 35te Tagung der Pathologen am Oberrhein/35th Meeting of Pathologists of the Upper Rhine Region (PATOR)

Open Access Oral presentation

Counterattack – a principle of tumour cell metastasation?

N Freudenberg1*, S Göppinger2, A Gold1, C Galanos3 and MA Feudenberg3

  • * Corresponding author: N Freudenberg

Author Affiliations

1 Institut für Pathologie der Universität Freiburg, Germany

2 Institut für Pathologie der Universität Heidelberg, Germany

3 Max-Planck-Institut für Immunbiologie Freiburg, Germany

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Diagnostic Pathology 2007, 2(Suppl 1):S25  doi:10.1186/1746-1596-2-S1-S25


The electronic version of this article is the complete one and can be found online at:


Published:14 March 2007

© 2007 Freudenberg et al; licensee BioMed Central Ltd.

Aims

The aim of the present study was to investigate whether tumour cell supernatants of 4 different malignant tumour cell lines with different aggressive behaviour show comparable pathobiological reactions.

Methods

Mature mouse (C57/Bl6) macrophages were incubated with the a.m. different tumour cell supernatants and followed by the investigation of apoptosis factors using immunocytochemistry. In addition, the experiments were performed with Fas-knock-out mouse macrophages and PCR in order to investigate the involvement of the Fas/FasL system.

Results

All tumour cell supernatants investigated induced a significant increase of apoptototic activities in macrophages compared with control groups. Interestingly, the tumour cell supernatants showed differences in their intensities of apoptosis-inducing effects on the macrophages one to each other. The Fas/FasL system has been identified as one of the involved factors from tumours which induce apotoses in macrophages.

Conclusion

Our observation of the induction of apoptoses in macrophages due to so far undefined tumour cell factors supports the counterattack hypothesis which supposes the active destruction of the tumoricidal cell system by neoplastic cells. For now, the counterattack is an exciting new way for research in tumour immunology and probably offers a therapeutic potential.