Positive color selection compared to CMYK analysis. (A) A representative field of 411 sections of cervical carcinoma shows CA-IX-DAB labeling with hematoxylin counterstain in full color and in Yellow intensity as shown in grayscale. (B) Percentages of IHC-labeled area as well as (C) mean intensity were analyzed using the Yellow channel and either results display a strong association with results obtained from positive color selection method.
Pham et al. Diagnostic Pathology 2007 2:8 doi:10.1186/1746-1596-2-8