STAT3 activation after delayed tissue fixation. (A) Phosphorylated STAT3-labeled AEC and hematoxylin counterstained representative images are shown in full color and Yellow intensity as shown in grayscale. (B) The mean Yellow intensity within the entire area of the four specimens shows a staining increase with a maximum at 20 minutes. This increase was smaller in magnitude and undetectable in results obtained from the normalized Red and the Key channel, respectively.
Pham et al. Diagnostic Pathology 2007 2:8 doi:10.1186/1746-1596-2-8