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Quantitative image analysis of immunohistochemical stains using a CMYK color model

Nhu-An Pham12, Andrew Morrison1, Joerg Schwock13, Sarit Aviel-Ronen1, Vladimir Iakovlev13, Ming-Sound Tsao123, James Ho1 and David W Hedley123*

Author Affiliations

1 Division of Applied Molecular Oncology, Ontario Cancer Institute/Princess Margaret Hospital, University Health Network, Toronto, Canada

2 Department of Medical Biophysics, University of Toronto, Toronto, Canada

3 Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada

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Diagnostic Pathology 2007, 2:8  doi:10.1186/1746-1596-2-8

Published: 27 February 2007

Additional files

Additional File 1:

Supplementary Figure 1 Chromogen spectral characteristics without hematoxylin counterstain. Color bars representing single IHC chromogens are defined by the individual color channels from the models CMYK, RGB, nRGB and HSL.

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Additional File 2:

Supplementary Figure 2 Activated STAT3 in tumor xenograft. Representative images of IHC staining for p-STAT3.

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