Methodology
Quantitative image analysis of immunohistochemical stains using a CMYK color model
1 Division of Applied Molecular Oncology, Ontario Cancer Institute/Princess Margaret Hospital, University Health Network, Toronto, Canada
2 Department of Medical Biophysics, University of Toronto, Toronto, Canada
3 Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada
Diagnostic Pathology 2007, 2:8 doi:10.1186/1746-1596-2-8
Published: 27 February 2007Additional files
Additional File 1:
Supplementary Figure 1 Chromogen spectral characteristics without hematoxylin counterstain. Color bars representing single IHC chromogens are defined by the individual color channels from the models CMYK, RGB, nRGB and HSL.
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Additional File 2:
Supplementary Figure 2 Activated STAT3 in tumor xenograft. Representative images of IHC staining for p-STAT3.
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