Table 1

The panel of immunohistochemical reagents used

Antibody

Clone

Manufacturer

Pre-treatment

Dilution


CK 7

OV-TL 12/30

Dako (Carpinteria, CA)

Pepsin for 10 minutes at 37°C

1/4000

CK 20

KS 20.8

Novocastra (Vision Biosystem, Norwell, MA)

Pepsin for 10 minutes at 37°C

1/50

Synaptophysin

27G12

Vector (Burlingame, CA)

HIER at pH 6.0

1/200

Chromogranin A

Polyclonal

Dako (Carpinteria, CA)

Pepsin for 10 minutes at 37°C

1/200

CD56

123C3

Zymed (Invitrogen, Carlsbad, CA)

HIER at pH 6.0

1/200

NSE

Polyclonal

Dako (Carpinteria, CA)

None

1/250

EGFR

31G7

Zymed (Invitrogen, Carlsbad, CA)

Pepsin for 10 minutes at 37°C

1/100

LMWK

Cam 5.2

B.D. Bioscience (San Jose, CA)

Pepsin for 10 minutes at 37°C

1/64

CK 19

6170

Novocastra (Vision Biosystem, Norwell, MA)

Pepsin for 10 minutes at 37°C

1/50

Pankeratin

AE1/AE3

Dako (Carpinteria, CA)

Pepsin for 10 minutes at 37°C

1/300

TTF-1

8G7G3/1

Neomarker (Lab Vision, Fremont, CA)

HIER at pH 6.0

1/800

CA 125

Ov185:1

Novocastra (Vision Biosystem, Norwell, MA)

HIER at pH 6.0

1:100

CDX2

Amt 28

Vision Biosystem (Norwell, MA)

HIER at pH 8.0

1/50

mCEA

B80-1

Biomeda (Foster City, CA)

None

1/200


HIER = Heat-induced epitope retrieval, in a Biocare decloaking chamber

El Demellawy et al. Diagnostic Pathology 2007 2:35   doi:10.1186/1746-1596-2-35

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