Table 1

Immunohistochemistry

Antibody

Expression in SPC

Expression in IMT

Source/Dilution


First stainings


Vimentin *

++

++

DAKO, mouse, 1:800

PanCytokeratin AE1/3 *

+

NR

DAKO, mouse, 1:100

EMA

+

+ (weak only)

LOXO, mouse, 1:20

ALK-1 *

NR

+

DAKO, mouse, 1:20

smooth muscle Actin

++

NR

Beckman Coulter, mouse, 1:20

Desmin

NR

NR

DAKO, mouse, 1:400

S100

NR

NR

DAKO, rabbit, 1:2000

CD34

NR

NR

DAKO, mouse, 1:100

CD117

NR

NR

DAKO, mouse, 1:100

Ki67 *

++ 60–80%

+ 5–10%

DAKO, mouse, 1:200


Additional stainings


PanCytokeratin KL1 *

+

NR

Beckman Coulter, mouse, 1:40

PanCytokeratin MNF116

NR (but epithelium +)

NR

DAKO, mouse, 1:50

Cytokeratin 5/6

+ (weak only)

NR

DAKO, mouse, 1:50

Cytokeratin 7

NR

NR

DAKO, mouse, 1:20

CD68

NR

NR

Kiel, ascites, 1:20000

CD30

NR

NR

DAKO, mouse, 1:5

CD56

NR

NR

DAKO, mouse, 1:10

Her2Neu

NR

NR

DAKO, rabbit, 1:100

Estrogen receptor

NR

NR

DAKO, mouse, 1:10

Progesteron receptor

NR

NR

DAKO, mouse, 1:10

p53

+

+

DAKO, mouse, 1:20

p63 *

++

NR

Neomarkers, mouse, 1:200

p21

++

++

Dianova, rat, 1:20

Cyclin D1

+

+

LOXO, mouse, 1:20

Bcl-2

+ (weak cytoplasmatic)

++

DAKO, mouse, 1:400

Rb

+

++

Neomarkers, mouse 1:50

HHV 8

NR

NR

Tebu, rat, 1:200

HPV

NR

NR

Virofem, mouse conc.


Used antibodies with expression pattern in spindle cell carcinoma (SPC) and inflammatory myofibroblastic tumor (IMT) as well as source and dilution. Staining started with antibodies in the upper part, followed by additional stainings, listed below. strong positive reaction (++), positive reaction (+), no reaction (NR), recommended for differential diagnosis (*).

Völker et al. Diagnostic Pathology 2007 2:1   doi:10.1186/1746-1596-2-1

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