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Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection

Torsten Goldmann1*, Renate Burgemeister2, Ulrich Sauer2, Siegfried Loeschke1, Dagmar Silvia Lang1, Detlev Branscheid3, Peter Zabel4 and Ekkehard Vollmer1

Author Affiliations

1 Clinical and Experimental Pathology, Research Center Borstel, Germany

2 P.A.L.M. Microlaser Technologies, Bernried, Germany

3 Department of Thoracic Surgery, Krankenhaus Großhansdorf, Germany

4 Medical Clinic, Research Center Borstel, Borstel, Germany

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Diagnostic Pathology 2006, 1:2  doi:10.1186/1746-1596-1-2

Published: 31 March 2006


As part of an investigation aimed at illuminating the possibilities and limits of the HOPE-fixation and paraffin-embedding technique we here describe a novel procedure which was developed in order to combine the benefits of the HOPE-technique with the capabilities of laser microdissection. The presented procedure avoids the need for amplification of template-RNA thus facilitating reliable and reproducible results. The excellent preservation of nucleic acids, proteins, and morphology in HOPE-fixed, paraffin-embedded tissues enhances the molecular applications available to date with materials acquired by laser microdissection when compared to formalin fixed, paraffin-embedded tissues, thus substantially extending the methodological panel in tissue based research.