Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection
1 Clinical and Experimental Pathology, Research Center Borstel, Germany
2 P.A.L.M. Microlaser Technologies, Bernried, Germany
3 Department of Thoracic Surgery, Krankenhaus Großhansdorf, Germany
4 Medical Clinic, Research Center Borstel, Borstel, Germany
Diagnostic Pathology 2006, 1:2 doi:10.1186/1746-1596-1-2Published: 31 March 2006
As part of an investigation aimed at illuminating the possibilities and limits of the HOPE-fixation and paraffin-embedding technique we here describe a novel procedure which was developed in order to combine the benefits of the HOPE-technique with the capabilities of laser microdissection. The presented procedure avoids the need for amplification of template-RNA thus facilitating reliable and reproducible results. The excellent preservation of nucleic acids, proteins, and morphology in HOPE-fixed, paraffin-embedded tissues enhances the molecular applications available to date with materials acquired by laser microdissection when compared to formalin fixed, paraffin-embedded tissues, thus substantially extending the methodological panel in tissue based research.