Table 3

UCCC scoring and interpretation of different types of EGFR gene amplification in NSCLC specimens.

Pattern Description

Scoring Criteria

Number of Cells to Score

Expected Ratio Gene to Chromosome

FISH result*


Large, loose clusters of red (EGFR) signals

Count signals thoroughly and calculate the FISH indexes

30

>2

GA

Small, loose clusters of red (EGFR) signals

Count signals thoroughly and calculate the FISH indexes

30

>2 or ~1 if high level of aneusomy 7 is present

GA if ratio >2 GA if clusters are present in >10% cells, independent of ratio

Large, loose co-localized clusters of red (EGFR) and green (CEP 7) signals

Count signals thoroughly, account for the innumerable signals using the symbol greater than (>) in front of the final counting

30

~1

GA if clusters are present in >10% cells

Tightly packed, innumerable cluster of red (EGFR) signals or atypically large red (EGFR) signal, consistently bigger than the green (CEP 7) signal in the tumor cells but smaller in the adjacent stromal and reactive cells

Count signals in at least 50 cells and identify each cell displaying this specific feature in the analysis sheet

≥ 50

~1

GA if tight clusters or atypical red signal is present in >10% cells

EGFR as double minutes

Count signals thoroughly and calculate the FISH indexes

30

>2

GA

Very high number of balanced red (EGFR) and green (CEP 7) signals

Count signals in at least 50 cells

≥50

~1

GA if >10% cells have ≥ 15 red (EGFR) signals


* GA = EGFR gene amplification

Varella-Garcia Diagnostic Pathology 2006 1:19   doi:10.1186/1746-1596-1-19

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