Table 1

Primers used for EBNA2 PCR typing

Primers
Sequence (5'-3')
Use in the PCR reaction
EBNA-2 Location*

EBNA-2F
TGGAAACCCGTCACTCTC
1st reaction sense
48572-89
EBNA-2I
TAATGGCATAGGTGGAATG
1st reaction sntisense
49355-73
EBNA-2C
AGGGATGCCTGGACACAAGA
Nested reaction sense
48810-29
EBNA-2G
GCCTCGGTTGTGACAGAG
Nested reaction antisense type-1
49048-65
EBNA-2B
TTGAAGAGTATGTCCTAAGG
Nested reaction antisense type-2
2020-39#

* Sequence locations for type-1 correspond to B95.8 coordinates (Accession number V01555) and for type-2 (#) to AG876 isolate (Accession number K03332).

Hassan et al. Diagnostic Pathology 2006 1:17   doi:10.1186/1746-1596-1-17