Sensitivity assays. Namalwa cells (2 EBV genome per cell) were serially diluted in the EBV-negative cells of Ramos cell line. PCR results showed that the method is able to detect 1 EBV genome in a background of 5 × 103 (first reaction) (A) and 1 × 104 negative cells (nested PCR) (B). 2.5% agarose gel stained with ethidium bromide.
Hassan et al. Diagnostic Pathology 2006 1:17 doi:10.1186/1746-1596-1-17