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Open Access Methodology

TMA for all: a new method for the construction of tissue microarrays without recipient paraffin block using custom-built needles

Andréa Rodrigues Cordovil Pires1*, Felipe da Matta Andreiuolo2 and Simone Rabello de Souza2

Author Affiliations

1 Fonte Medicina Diagnóstica Ltda. and Fluminense Federal University, Rio de Janeiro, Niterói, Brazil

2 Fonte Medicina Diagnóstica Ltda., Rio de Janeiro, Niterói, Brazil

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Diagnostic Pathology 2006, 1:14  doi:10.1186/1746-1596-1-14

Published: 25 July 2006

Abstract

Background

TMAs are becoming a useful tool for research and quality control methods, mostly for immunohistochemistry and in situ hybridization.

Methods

A new technique that allows building TMA blocks with more than 300 tissue cores without using a recipient paraffin block for the tissue cores and without using a commercial TMA builder instrument is described. This technique is based on the construction of TMA needles modifying conventional hypodermic needles to punch tissue cores from donor blocks, which are attached by double-side adhesive tape on a computer-generated paper grid used to align the cores on the block mould, which is filled with liquid paraffin.

Results

More than two hundred TMA blocks were constructed using this method, utilized in immunohistochemistry and histochemistry as positive and negative controls and also in research.

Conclusion

This technique has the following advantages: it is easy to reproduce, affordable, quick and creates uniform blocks with more than 300 cores aligned, adherent and easy to cut, with negligible losses during cutting and immunohistochemistry and in situ hybridization procedures.